|Standard Curve Range||0.78125 ng/mL-200 ng/mL|
|Suitable Sample Type||For the quantitative determination of human Soluble ROR1 in Cell Culture Supernatants, Plasma, Serum.|
|Sample volume||50 μL|
|CEA050-B01||Pre-coated Anti-ROR1 Antibody Microplate||1 plate|
|CEA050-B02||Human ROR1 Standard||20 μg×2|
|CEA050-B03||Biotin-Anti-ROR1 Antibody Con. Solution||400 μL|
|CEA050-B04||Biotin-Antibody Dilution Buffer||8mL|
|CEA050-B05||Streptavidin-HRP Con. Solution||500 μL|
|CEA050-B06||Streptavidin-HRP Dilution Buffer||15 mL|
|CEA050-B07||20× Washing Buffer||50 mL|
|CEA050-B08||Sample Dilution Buffer||15 mL ×2|
|CEA050-B09||Substrate Solution||12 mL|
|CEA050-B10||Stop Solution||6 mL|
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human ROR1 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of human ROR1 in serum, plasma and cell culture supernates.
It is for research use only.
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of ROR1 is less than 0.78125 pg/mL.
Ten replicates of each of 4 samples containing different ROR1 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Five samples containing different concentrations of ROR1 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant ROR1 was spiked into 3 human serum samples, and then analyzed. The average recovery of ROR1 for serum samples is 103.2%.
Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development.