|Standard Curve Range||3.906 pg/mL-500 pg/mL|
|Assay Time||2 hr|
|Suitable Sample Type||For the quantitative determination of humanGranzyme B in Cell Culture Supernatants, Plasma, Serum.|
|Sample volume||50 μL|
|CEA033-C01||Pre-coated Anti- Granzyme B Antibody Microplate||1 plate|
|CEA033-C02||Human Granzyme B Standard||20 μL|
|CEA033-C03||Biotin-Anti- Granzyme B Antibody Con. Solution||100 μL|
|CEA033-C04||Biotin-Antibody Dilution Buffer||8 mL|
|CEA033-C05||Streptavidin-HRP Con. Solution||0.5 mL|
|CEA033-C06||Streptavidin-HRP Dilution Buffer||15 mL|
|CEA033-C07||20× Washing Buffer||50 mL|
|CEA033-C08||Sample Dilution Buffer||15 mL ×2|
|CEA033-C09||Substrate Solution||12 mL|
|CEA033-C10||Stop Solution||6 mL|
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human Granzyme B that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of human Granzyme B in serum, plasma and cell culture supernates.
It is for research use only.
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of Granzyme B is less than 1 pg/mL.
Granzyme B (GrB) is one of the serine protease granzymes most commonly found in the granules of natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells.