|Standard Curve Range||62.5 pg/mL-8000 pg/mL|
|Assay Time||1 hr 45 min|
|Suitable Sample Type||For the quantitative determination of human HGF in Cell Culture Supernatants, Plasma, Serum.|
|Sample volume||50 uL|
|CRB012-C01||Pre-coated Anti-HGF Antibody Microplate||1 plate|
|CRB012-C02||Human HGF Standard||20 μg×2|
|CRB012-C03||Biotin-Anti-HGF Antibody Con. Solution||100 μL|
|CRB012-C04||Biotin-Antibody Dilution Buffer||8 mL|
|CRB012-C05||Streptavidin-HRP Con. Solution||500 μL|
|CRB012-C06||Streptavidin-HRP Dilution Buffer||15 mL|
|CRB012-C07||20× Washing Buffer||50 mL|
|CRB012-C08||Sample Dilution Buffer||15 mL×2|
|CRB012-C09||Substrate Solution||12 mL|
|CRB012-C10||Stop Solution||6 mL|
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human HGF that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of human HGF in serum, plasma and cell culture supernates.
It is for research use only.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
This assay kit is used to measure the concentrations of human HGF by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- HGF Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-HGF Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of HGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of HGF is less than 40.0 pg/mL.
Hepatocyte growth factor, HGF
Hepatocyte growth factor (HGF) is a paracrine cellular growth, motility and morphogenic factor. It is secreted by mesenchymal cells and targets and acts primarily upon epithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells and T cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration, and in wound healing.