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产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | IFN-γ |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | 15 pg/mL |
| Standard Curve Range | 15.625 pg/mL-1000 pg/mL |
| Assay Time | 1 hr 15 min |
| Suitable Sample Type | For the quantitative determination of human IFN-γ in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
组分（Materials Provided）
| ID | Components | Size |
| CRB013-C01 | Pre-coated Anti-IFN-γ Antibody Microplate | 1 plate |
| CRB013-C02 | Human IFN-γ Standard | 14 μg×2 |
| CRB013-C03 | Biotin-Anti- IFN-γ Antibody Con. Solution | 100 μL |
| CRB013-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CRB013-C05 | Streptavidin-HRP Con. Solution | 500μL×2 |
| CRB013-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CRB013-C07 | 20× Washing Buffer | 50 mL |
| CRB013-C08 | 1× Dilution Buffer | 15 mL×2 |
| CRB013-C09 | Substrate Solution | 12 mL |
| CRB013-C10 | Stop Solution | 6 mL |
背景（Background）
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IFN-γ that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
应用说明（Application）
The kit is developed for quantitative detection of human IFN-γ in serum, plasma and cell culture supernates.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit is used to measure the concentrations of human Interferon Gamma (IFN-γ) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IFN-γ Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IFN-γ Antibody and HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRB013-C02 is less than 15.0 pg/mL.
背景（Background）：IFN-gamma
Interferon gamma, Interferon-γ
Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFN-gamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.
Cytokine storm syndromes is an activation cascade of pro-inflammatory cytokines, such as TNF-α, IL-1, IL-6, IL-12, IFN-α, IFN-β, IFN-γ, MCP-1 and IL-8, due to unregulated host immune response system to different stimuli such as infections, malignancy, rheumatoid disorders, drugs, and so on. Interferon-γ (IFN-γ), one of the four subclasses ofinterferons, and is a multipotent protein, acting on many cell types by inducing or inhibiting many cellular functions through direct effects on gene expression.
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