|Standard Curve Range||4.69 pg/mL-600 pg/mL|
|Assay Time||2 hr|
|Suitable Sample Type||For the quantitative determination of human IL-1β in serum, plasma and cell supernatant.|
|Sample volume||50 μL|
|CRB002-C01||Pre-coated Anti-IL-1β Antibody Microplate||1 plate|
|CRB002-C02||Human IL-1β Standard||20 μg×2|
|CRB002-C03||Biotin-Anti-IL-1β Antibody Con. Solution||100 μL|
|CRB002-C04||Biotin-Antibody Dilution Buffer||8 mL|
|CRB002-C05||Streptavidin-HRP Con. Solution||500 μL|
|CRB002-C06||Streptavidin-HRP Dilution Buffer||15 mL|
|CRB002-C07||20× Washing Buffer||50 mL|
|CRB002-C08||1× Dilution Buffer||15 mL×2|
|CRB002-C09||Substrate Solution||12 mL|
|CRB002-C10||Stop Solution||6 mL|
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-1β that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of IL-1beta in human serum and cell culture supernates.
It is for research use only.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
This assay kit is used to measure the concentrations of human Interleukin 2 (IL-1β) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-1β Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-1β Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B002 is less than 1.0 pg/mL.
IL-1β is a potent pro-inflammatory cytokine. IL-1β is produced and secreted by various cell types, such as immune cells, fibroblasts, or cancer cells. The IL-1β gene is induced after "priming" of the cells and a second signal is required to allow IL-1β maturation by inflammasome-activated caspase-1. IL-1β is then released and leads to transcription of target genes through its ligation with IL-1R1 on target cells. IL-1β expression and maturation are guided by gene polymorphisms and by the cellular context. In cancer, IL-1β has pleiotropic effects on immune cells, angiogenesis, cancer cell proliferation, migration, and metastasis.