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产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | IL-6 |
| Format | 96T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | <2.0 pg/mL |
| Standard Curve Range | 6.25 pg/mL-400 pg/mL |
| Assay Time | 2 hr |
| Suitable Sample Type | For the quantitative determination of human IL-6 in serum, plasma and cell supernatant. |
| Sample volume | 50 μL |
组分（Materials Provided）
| ID | Components | Size |
| CRB001-C01 | Pre-coated Anti-IL-6 Antibody Microplate | 1 plate |
| CRB001-C02 | Human IL-6 Standard | 20 μg×2 |
| CRB001-C03 | Biotin-Anti-IL-6 Antibody Con. Solution | 100 μL |
| CRB001-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CRB001-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CRB001-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CRB001-C07 | 20× Washing Buffer | 50 mL |
| CRB001-C08 | 1× Dilution Buffer | 15 mL×2 |
| CRB001-C09 | Substrate Solution | 12 mL |
| CRB001-C10 | Stop Solution | 6 mL |
背景（Background）
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-6 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
应用说明（Application）
The kit is developed for quantitative detection of IL-6 in human serum and cell culture supernates.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储（Storage）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
原理（Assay Principles）
This assay kit is used to measure the concentrations of human Interleukin 6 (IL-6) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-6 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-6 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B001 is less than 2.0 pg/mL.
背景（Background）：IL-6
Interleukin 6, IL-6
Interleukin-6 (IL-6) is a member of the pro-inflammatory cytokine family. It induces the expression of a variety of proteins responsible for acute inflammation, and plays an important role in the proliferation and differentiation of cells in humans. It has been demonstrated the dysregulation of the IL-6 signaling pathways can result in inflammatory and autoimmune disorders as well as cancer development.
Human IL-6 (also known as B-cell stimulatory factor 2, Interferon beta-2, Hybridoma growth factor, and CTL differentiation factor) plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth, nerve cells differentiation, and acute phase reactants in hepatocytes. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells, and keratinocytes. It has been shown that IL-6 plays a critical role in many physiological and pathological conditions, including autoimmune diseases and rheumatoid arthritis.
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