|Standard Curve Range||7.81 pg/mL-1000 pg/mL|
|Assay Time||2 hr|
|Suitable Sample Type||For the quantitative determination of human IL-4 in serum, plasma and cell supernatant.|
|Sample volume||50 μL|
|CRB003-C01||Pre-coated Anti-IL-4 Antibody Microplate||1 plate|
|CRB003-C02||Human IL-4 Standard||20 μg×2|
|CRB003-C03||Biotin-Anti-IL-4 Antibody Con. Solution||100 μL|
|CRB003-C04||Biotin-Antibody Dilution Buffer||8 mL|
|CRB003-C05||Streptavidin-HRP Con. Solution||500 μL|
|CRB003-C06||Streptavidin-HRP Dilution Buffer||15 mL|
|CRB003-C07||20× Washing Buffer||50 mL|
|CRB003-C08||1× Dilution Buffer||15 mL×2|
|CRB003-C09||Substrate Solution||12 mL|
|CRB003-C10||Stop Solution||6 mL|
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IL-4 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of IL-4 in human serum and cell culture supernates.
It is for research use only.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
This assay kit is used to measure the concentrations of human Interleukin 4 (IL-4) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- IL-4 Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-IL-4 Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B003 is less than 6.0 pg/mL.
IL-4, Interleukin 4
IL-4 is a cytokine with pleiotropic functions. IL-4 plays a key role in the development of Th2 responses. In mice that lack IL-4, there is significant abrogation of Th2 cellular activity. IL-4 promotes the growth and activation of B lymphocytes that produce IgG1 and IgE classes of antibodies and increases MHC class II induction. Preclinical animal models have demonstrated modest antitumor efficacy for IL-4. In the nonimmunogenic B16 murine melanoma model, irradiated B16 cells engineered to express IL-4 induced antitumor protection against a wild-type B16 challenge.