|Standard Curve Range||19.5 pg/mL-1250 pg/mL|
|Assay Time||2 hr 50 min|
|Suitable Sample Type||For the quantitative determination of human IFN-γ in Cell Culture Supernatants, Plasma, Serum.|
|Sample volume||100 μL|
|CRS017-C01||Pre-coated Anti-IFN-γ Antibody Microplate||1 plate|
|CRS017-C02||Human IFN-γ Standard||30 μg|
|CRS017-C03||Biotin-Anti-IFN-γ Antibody||25 μg|
|CRS017-C05||10xWashing Buffer||50 mL|
|CRS017-C06||2xDilution Buffer||50 mL|
|CRS017-C07||Substrate Solution||12 mL|
|CRS017-C08||Stop Solution||7 mL|
ClinMax? ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human IFN-gamma that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax? ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of IFN-γ in human serum and cell culture supernates.
It is for research use only.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储 & 运输（Storage & Shipping）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Human IFN-γ. The kit consists of Pre-coated Anti-IFN-γ Antibody Microplate and Human IFN-γ Standard and Biotin-Anti-IFN-γ Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20°C-25°C) before use.
b) Add your sample to the plate and take the Human IFN-γ as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-IFN-γ Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, each ELISA plate needs to set the standard curve. The following CRS-A017 standard curve data is for reference only.
Interferon gamma, Interferon-γ
Interferon-gamma (IFN-γ/IFNG) is a dimerized soluble cytokine that is the only member of the type II class of interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-gamma has been used in a wide variety of clinical indications. Interferon-gamma (IFNgamma) is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway. Interferon gamma has broader roles in activation of innate and adaptive immune responses to viruses and tumors, in part through upregulating transcription of genes involved in cell cycle regulation, apoptosis, and antigen processing/presentation. Despite this, rodent and human trophoblast cells show dampened responses to IFNG that reflect the resistance of these cells to IFNG-mediated activation of major histocompatibility complex (MHC) class II transplantation antigen expression.
Cytokine storm syndromes is an activation cascade of pro-inflammatory cytokines, such as TNF-α, IL-1, IL-6, IL-12, IFN-α, IFN-β, ,IFN-γ, MCP-1 and IL-8, due to unregulated host immune response system to different stimuli such as infections, malignancy, rheumatoid disorders, drugs, and so on. Interferon-γ (IFN-γ), one of the four subclasses ofinterferons, and is a multipotent protein, acting on many cell types by inducing or inhibiting many cellular functions through direct effects on gene expression.