|RAB001-C01||High-bind Plate||1 plate|
|RAB001-C02||Mouse FMC63 scFv||25 μg|
|RAB001-C03||FMC63 ADA Standard||15 μg|
|RAB001-C04||Biotin-Mouse FMC63 scFv||20 μg|
|RAB001-C06||Coating Buffer||12 mL|
|RAB001-C07||10xWashing Buffer||50 mL|
|RAB001-C08||Blocking Buffer||50 mL|
|RAB001-C09||Substrate Solution||12 mL|
|RAB001-C10||Stop Solution||7 mL|
FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.Anti-fmc63 scFv antibody can specifically bind to the antigen recognition epitope of fmc63 scFv on anti-CD19 car, and it shows high specificity and sensitivity,it's used to detect the expression of fmc63 scFv derived car.
This kit is developed for detection of Anti-CD19 (FMC63) antibodies in CAR Immunogenicity assay test. Detection and monitoring the level of ADA is important to fully understand the potential immune responses.
It is for research use only.
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储 & 运输（Storage & Shipping）
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
This assay kit employs a bridging-ELISA format, providing a rapid detection of FMC63 ADA. The kit consists High-bind Plate and Mouse FMC63 scFv and FMC63 ADA Standard and Biotin-Mouse FMC63 scFv and Streptavidin-HRP and buffers.
Your experiment will include 4 simple steps:
a) Coat the plate with Mouse FMC63 scFv.
b) Add your molecule of interest to the tests.
c) Add Mouse FMC63 scFv-Biotin.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
典型数据-Typical Data Please refer to DS document for The assay protocol.
Detection of FMC63 ADA titer by bridging-ELISA Assay.
Immobilized Mouse FMC63 scFv can bind FMC63 ADA in 1:20 human serum, and then add Biotin-Mouse FMC63 scFv. Detection was performed using HRP-conjugated streptavidin with sensitivity of 19 ng/mL (QC tested).