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产品描述（Product Details）
| Assay Type | Sandwich-ELISA |
| Analyte | Nuclease |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | < 39 pg/mL |
| Standard Curve Range | 39.06 pg/mL-2500 pg/mL |
| Assay Time | 3 hr 20 min |
| Suitable Sample Type | For the quantitative determination the residue of nuclease in biologicals. |
| Sample volume | 100 μL |
组分（Materials Provided）
| ID | Components | Size |
| CRS016-C01 | Pre-coated Anti-Nuclease Antibody Microplate | 1 plate |
| CRS016-C02 | Nuclease Standard | 50 μL |
| CRS016-C03 | Biotin-Anti-Nuclease Antibody | 100 μL |
| CRS016-C04 | Streptavidin-HRP | 50 μL |
| CRS016-C05 | 10xWashing Buffer | 50 mL |
| CRS016-C06 | 2xDilution Buffer | 50 mL |
| CRS016-C07 | Substrate Solution | 12 mL |
| CRS016-C08 | Stop Solution | 7 mL |
背景（Background）
GENIUS?Nuclease ELISA Kit is based on ELISA sandwich method and designed for quantitative determination the residue of nuclease in biologicals. It contains Nuclease and a pair of antibodies against the eneyme, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of nuclease concentrations.
应用说明（Application）
The GENIUS?ELISA Kit was developed for the detection and quantitative determination of nuclease in samples from downstream processing where nuclease is used as a process or purification aid.
The ?limitation of detectable dose (LOD) of Cat. No. CRS-A016?was determined as 2.733 pg/mL.
It is for research use only.
重构方法（Reconstitution）
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
存储 & 运输（Storage & Shipping）
Unopened kit should be stored at 2°C -8°C upon receiving.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
原理（Assay Principles）
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of Nuclease. The kit consists of Pre-coated Anti-Nuclease Antibody Microplate and Nuclease Standard and Biotin-Anti-Nuclease Antibody and Streptavidin-HRP and buffers.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the Nuclease as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Add a diluted Biotin-Anti-Nuclease Antibody to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
典型数据-Typical Data Please refer to DS document for The assay protocol.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
GENIUS? Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A016) can detect the nuclease from different manufacturers with similar sensitivity.
GENIUS? Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A016) can detect the nuclease in different culture supernatant with similar curve and sensitivity.
GENIUS? Nuclease ELISA Kit (Residue Testing) (Cat. No. CRS-A016) can detect the nuclease from different culture supernatant and was free from the matrix effects. * means a significant difference compared with the negative, NS means no significant difference with the negative.
验证（Validation）
精密度（Precision）
Intra-Assay Precision : Twenty replicates of each of three samples containing different concentrations were tested in one assay. Within the detection range, the CV is less than 10%.
Inter-Assay Precision : Three replicates of each of three samples containing different concentrations were tested in three independent assays. Within the detection range, the CV is less than 10%.
回收率（Recovery）
Three parts of blank medium were added with three different concentrations of Nuclease, and the medium without Nuclease was used as background to calculate the recovery rate.
背景（Background）：Nuclease
Nucleases are enzymes that degrade nucleic acids, either DNA or RNA. It has been used for the preparation of nuclear extracts by digesting DNA and releasing nuclear proteins intimately associated with DNA. It has also been designed for removing RNA and DNA in biotechnological processing. Thus, the amount of residual nuclease in biological products should be detected and limited.
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